History
Array CGH

Choose platform

First, 3 kinds of large-insert clones

i. YACs (yeast artificial chromosomes)

ii. BACs (bacterial artificial chromosomes)

iii. PACs (P1-deriver artificial chromosomes) 

Later, choose shorten platform:

Cosmids and fosmids clones.

After that, they used cDNA platform.

Finally, the oligonucleotide array CGH (oaCGH) has genome-covering resolution be chosen. (Bauke, 2006).

By the way, this page introduced the BACs and oaCGH arrays.

Introduction to Array CGH
Compare
Content
Example
Reference website
Reference theses
Message
homeHome

BACs (bacterial artificial chromosomes)

        It is the first acquainted genomic arrays which has low sensitivity, but used to detect the single copy of the genome. The BAC clones for array CGH, because of BACs has large size, so it invested a lot of time and expensive, limits its resolution. 

                                                                                                                                   TOP

OaCGH (oligonucleotide array CGH)

       Permit the flexible probe design, and has larger coverage, higher resolution. The higher resolution depends on array design and the homogeneous cell type. In addition, oaCGH is easy to produce by any organism in which the genome has been sequenced. Nowadays, oaCGH is usually in common used.

       For the instances, compare with BACs and oaCGH in “compare” site, it has many article examples in there.
                                                                                                                                   TOP

       The usefulness of oligonucleotide arrays for aCGH could reduce complexity

       To investigate and develop probe-design criteria, assay conditions, and analysis methods that enable 60-mer oligonucleotide arrays to be used for CGH measurements even when using total genomic DNA.